Mouse NADH Dehydrogenase, Quinone 1 (NQO1) ELISA Kit

FOR RESEARCH USE ONLY. Not for clinical DIAGNOSIS USE CATALOG #:B1558 For the quantitative determination of Mouse NQO1 concentrations.  Detection of species: Mouse Detection medium: serum, plasma, tissue homogenates, cell culture supernates


This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated
with an antibody specific to NQO1. Standards or samples are added to the appropriate Microelisa stripplate wells and
combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for NQO1 is added to
each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to
each well. Only those wells that contain NQO1 and HRP conjugated NQO1 antibody will appear blue in color and then turn
yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength
of 450 nm. The OD value is proportional to the concentration of NQO1. You can calculate the concentration of NQO1 in the
samples by comparing the OD of the samples to the standard curve.


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