Description
Mouse Melatonin,MT ELISA Kit
The kit is for the quantitative level of melatonin in the sample, adopt purified Mouse melatonin to coat microtiter plate,
make solid-phase antibody, then add melatonin to wells, Combine melatonin antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate
becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of melatonin in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
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