Human heparin-binding epidermal growth factor-like growth factor,HB-EGF ELISA Kit
The kit is for the quantitative level of HBEGF in the sample, adopt purified human HBEGF to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to HBEGF, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of HBEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.