Rat Collagen Type Ⅰ(Col I) ELISA Kit
The kit is for the quantitative level of Col I in the sample, adopt purified Rat Col I to coat microtiter plate, make solid-phase
antibody, then add Col I to wells, Combine Col I antibody with labeled HRP to form antibody-antigen -enzyme-antibody
complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP
enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of Col I in the samples is then determined by comparing the O.D. of the samples
to the standard curve.