The kit is for the quantitative level of P-p53 in the sample, adopt purified Mouse P-p53 to coat microtiter plate, make
solid-phase antibody, then add P-p53 to wells, Combine P-p53 antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of P-p53 in the samples is then determined by comparing the O.D. of the samples
to the standard curve.