Description
Human leukotriene D4,LT-D4 ELISA Kit
The kit is for the quantitative level of LTD4 in the sample, adopt purified human LTD4 to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to LTD4, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of LTD4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Reviews
There are no reviews yet.