Human Leptin Soluble Receptor,sLR ELISA Kit
The kit is for the quantitative level of LEPR in the sample, adopt purified human LEPR to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to LEPR, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody – antigen – enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of LEPR in the samples is then determined by comparing the O.D. of the samples to the standard curve.