The kit is for the quantitative level of FGF19 in the sample, adopt purified Monkey
FGF19 to coat microtiter plate, make solid-phase antibody, then add samples or
standards to wells with a labeled antibody specific to FGF19, then add labeled HRP to
the well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody – antigen – enzyme-antibody
complex, reaction is terminated by the addition of a stop solution and the color change
is measured at a wavelength of 450 nm. The concentration of FGF19 in the samples is
then determined by comparing the O.D. of the samples to the standard curve.