1, 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1), Human, ELISA Kit

Description

1, 2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1), Human, ELISA Kit

The kit is for the quantitative level of ADI1 in the sample, adopt purified human ADI1 to
coat microtiter plate, make solid-phase antibody, then add samples or standards to
wells with a labeled antibody specific to ADI1, then add labeled HRP to the well. After
washing completely, add TMB substrate solution, TMB substrate becomes blue
color in wells that contains antibody – antigen – enzyme-antibody complex, reaction
is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of ADI1 in the samples is then determined by
comparing the O.D. of the samples to the standard curve.